Conditions for the multiplex PCR with HDP2-based primers were 94°C for 5 min (initial denaturation), followed by 35 cycles at 94°C for 1 min, 56.5°C for 30 s, 72°C for 30 s, and 72°C for 10 min (final extension). Thus, the PCR with HDP1-based primers offers the possibility of a sensitive, rapid, and specific method for the reliable identification of T. saginata in the absence of a signal from T. solium and other taeniids. Taenia saginata infestation has got a global distribution and is endemic in this part of the world. Only human are the definitive hosts for Taenia solium, Taenia saginata, and Taenia asiatica, which are referred as the human-Taenia.The distribution of each of the 3 species of human Taenia depends on peoples’ cultural characteristics which involve the consumption of uncooked meat or organs of intermediate hosts infected with viable metacestodes [1–3]. 359 (1-2) Escobedo, A. The locations of the PTs7S35F1, PTs7S35F2, and PTs7S35R1 oligonucleotide primers are indicated by arrows. Adult worm lives in the small intestine (upper jejunum) of human.Systematic […] solium, T. saginata, and Echinococcus granulosus. The reactions were carried out as described in Materials and Methods. This is the unarmed tapeworm of man causing taeniasis and it can cause cysticercosis in cattle. Taenia saginata. Design of PCR primers derived from HDP1 and HDP2 sequences. Electrophoresis, Southern blotting, labeling, and hybridization procedures. Possibly some mechanism analogous to similar previously described mechanisms acting on the satellite DNA was responsible (7, 18, 28, 41, 42). Taenia saginata was differentiated from Taenia solium infection by the late 1700s. Indeed, the specificity of the T. saginata 1,272-bp HDP1 target sequence is exquisite, as Harrison et al. Taeniasis, caused by two major Taenia species, T. solium and T. saginata, is a worldwide foodborne zoonotic disease.T. Samples of genomic DNA fromT. To our knowl-edge, this report is the fi rst of T. asiatica and a dual Taenia infection from Thailand. This work was supported by grants from UE-INCO (grant DCIC 18CT950002), FISS (grant 97/0141), and MEC/British Council (grant HB96-43). The restriction enzyme recognition sites are indicated by the lines. First, 3-μg aliquots of T. saginata gDNA were digested to completion with different restriction endonucleases (Amersham Life Science, Buckinghamshire, England; Boehringer Mannheim GmbH, Mannheim, Germany; Promega Corporation) by following the procedures recommended by the manufacturers. The amplification products were fractionated on a 2% agarose gel and were stained with ethidium bromide. Taenia saginata Y Taenia solium. The zoonotic tapeworms Taenia saginata and solium like their definitive host (humans) have a global distribution and wid ecological niche, T. saginata being recorded on every continent with the exception of Antarctica and T. solium being recorded in the majority of countries where pork is consumed. Electrophoresis of the digested DNA samples and subsequent transfer to positively charged nylon membranes (Boehringer Mannheim GmbH) were carried out by standard procedures (40). The areas with the highest prevalence (up to 27%) are in central Asia, the Near East, and Central and East Africa. Direct and inverted internal repeats are indicated by arrows above and below the consensus sequence (con), respectively. saginata HDP1 probe. Elles seront également utilisées sous réserve des options souscrites, à des fins de ciblage publicitaire. The morphologic characteristics of these two parasites are very similar. Diagnosis, treatment and control of cysticercosis by Taenia solium. We thank J. M. Rubio for technical help in the design of the PCR with HDP1-based primers and L. Benı́tez and E. Rodrı́guez for helpful discussions. Samples of genomic DNA (10 ng) from T. saginata (lane 1), T. solium (lane 2), T. taeniformis B (lane 3), T. taeniformis M (lane 4), E. granulosus (lane 5), a calf (lane 6), and a human (lane 7) were amplified with the PTs4F1 and PTs4R1 primers as described in Materials and Methods. Unless molecular diagnosis is carried out, the tapeworm could belong to T. saginata … 1752 N St. NW This organism also developed into tapeworms in chacma The digested gDNAs were probed with three nonoverlapping fragments derived from the HDP2 sequence fragments: 5PHDP2 (A), IPHDP2 (B), and 3PHDP2 (C). Regions reporting T. solium infections in the porcine or human hosts include Africa (Braae et al., 2015) and Asia (Rajshekhar et al., 2003), Latin America (Coral-Almeida et al,. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. saginata. solium is found in people who habitually eat raw or undercooked pork, while T. saginata is found in people who habitually eat raw or undercooked beef. After hybridization, the filters were washed at 68°C for 10 min in 2× SSC–0.1% sodium dodecyl sulfate (SDS) and then for a further 40 min in 0.1× SSC–0.1% SDS. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to bothT. Hybridizations were conducted overnight under high-stringency conditions at 68°C. Primer PTs7S35F1 was based on the 5PHDP2 sequence, and primers PTs7S35F2 and PTs7S35R1 were designed from the IPHDP2 sequence (Fig.6B). Background. It is the armed tapeworm of human. The scolex of T. solium contains four large suckers and a rostellum containing two rows of large and small hooks. Taenia saginata: Its growth and propagation - Volume 15 Issue 1 - W. J. Penfold, H. Boyd Penfold, Mary Phillips 8). Taenia saginata, known as the beef tapeworm, is transmitted to humans in the form of infectious larval cysts found in the meat of cattle, which serve as the parasite's usual intermediate host. Immunodiagonosis of taeniasis by coproantigen detection, A tandemly repetitive DNA sequence is present at diverse locations in the genome of, Isolation and characterization of species-specific DNA probes from, Distribution and sequence of an abundant satellite DNA in the beetle Tenebrio molitor, An enzyme-linked immunosorbent assay for diagnostic detection of, A comprehensive set of sequence analysis programmes for the VAX, Characterization of a species-specific satellite DNA from the entomopathogenic nematode Steinernema carpocapsae, Molecular characterization of two species-specific tandemly repeated DNAs from entomopathogenic nematodes, Repetitive sequences in eukaryotic DNA and their expression, Structure, evolution and properties of a novel repetitive DNA family in, Isolation and characterization of a middle repetitive DNA element from, Human cysticercosis and taeniasis: molecular approaches for specific diagnosis and parasite identification, Cloning and characterization of two satellite DNAs in the low-C-value genome of the nematode, Curvature of mouse satellite DNA and condensation of heterochromatin, DNA hybridization probe for clinical diagnosis of, Detection of specific sequences among DNA fragments separated by gel electrophoresis. Life cycles 5 1.3. Several proglottids expelled after medication with praziquantel were morphologically and molecularly confirmed to be T. saginata tapeworms. Mesures up to 10 metres. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg. It is globular in shape and has 4 circular suckers. The aim of this work was to standardize a method to obtain T.solium PO forms by in vitro cultivation. A negative control without DNA was also included (lane 9). The 53-bp monomer sequence was remarkably conserved in comparison to other repetitive sequences from parasite DNA that have been described (18, 42), with only 0.3% divergence among the 24 sequenced units and with only four base changes from the 53-bp consensus sequence. The potential diagnostic properties of this modified PCR protocol were evaluated with purified gDNAs from T. saginata, T. solium, and other related cestodes. We do not retain these email addresses. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. It is found amongst beef eaters all over the world. Southern blotting was done with T. saginata (lanes 1) and T. solium(lanes 2) gDNAs (5 μg) cleaved with the ClaI restriction enzyme. NAME: Taenia saginata. Taenia solium infections (taeniasis/cysticercosis) are a major scourge to most developing countries. In addition, T. solium eggs can infect humans, often giving rise to fatal neurocysticercosis (12, 39). Repetitive T. saginata HDP1 sequence (1,272 bp). Substitution point mutations are indicated by an arrow below each mutation. Infections with these cestodes are therefore a serious public health problem in areas of endemicity. We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia saginata and Taenia solium. Strikingly, the same adenine-to-guanine transition occurred at nucleotide 19 in three of the motifs located in the 4th, 7th, and 17th positions within the sequence. In order to identify unique sequences of HDP2 that do not occur in theT. To our knowl-edge, this report is the fi rst of T. asiatica and a dual Taenia A negative non-DNA containing-control was also included (lane 8). Sensitivity of PCR amplification with HDP1-based primers. Les informations recueillies sont destinées à CCM BENCHMARK GROUP pour vous assurer l'envoi de votre newsletter. HDP1 and HDP2 sequencing.For sequencing, the HDP2 DNA fragment was directly subcloned from λgt10 phage into the pBluescript SK+ plasmid, and then 25 nested deleted clones were selected and sequenced. saginata, and E. granulosus. These data demonstrated the T. saginata species specificity of the PTs7S35F1-PTs7S35R1 primer combination on the 600-bp target sequence, as well as the T. saginata and T. soliumspecificity of the PTs7S35F2-PTs7S35R1 primer combination on the 150-bp target sequence. 1). The clearest results were obtained at an annealing temperature of 56.5°C (data not shown). An evaluation of the genomic organization by Southern blot analysis, restriction enzyme mapping, and sequencing indicated that the 53-bp monomers were arranged in an estimated 11,321 clustered tandem repeats along 23 kb or more of theT. With amounts of T. saginata and T. solium DNA in the 10- to 40-ng range, the ladder amplification was observed only with T. saginataDNA (Fig. PDF | Background Taeniasis caused by two major Taenia species, T. solium and T. saginata is a food borne zoonotic disease of man worldwide. Genomic organization of HDP1 and HDP2 probes in T. saginata genome.In order to study the genomic organization of the HDP1 and the HDP2 sequences, taeniid gDNA was digested to completion with several restriction enzymes, transferred to membranes, and hybridized with both HDP1 and HDP2 under high-stringency conditions. Conventional coproscopical examination has a low specificity and sensitivity (29), whereas coproantigen detection by enzyme-linked immunosorbent assay, although sensitive, suffers from poor specificity due to cross-reactions with other taeniids and related helminths (1, 8, 23, 24). The use of DNA probes, as successfully used for species-specific detection of various parasites (2, 11, 14, 35, 37, 44), including T. solium and T. saginata (5, 13, 19, 32), is time-consuming and relatively insensitive. T ... Morphology. Taenia saginata, T. solium and T. asiatica (cysticercosis) Humans are the definitive hosts for T. saginata, T. solium and T. asiatica. SYNONYM OR CROSS REFERENCE: Beef tapeworm Footnote 1, Footnote 2, cysticercosis Footnote 1, taeniasis saginata Footnote 3, Cysticercus bovis Footnote 3.. CHARACTERISTICS: Taenia saginata is a tapeworm of the class cestoidea, order cyclophyllidea, and … egg contains approximately 8 pg of gDNA (32), the PCR should be able to detect the gDNA from oneT. Morphology of Taenia saginata. Morphology 1 1.2. The restriction enzyme sites are indicated by lines, and the PTs4F1 and PTs4R1 oligonucleotide primers are indicated by arrows. Background. Un taenia saginata adulte possède un corps en forme de ruban, comportant de l'ordre de 500 à 2000 anneaux et une tête équipée de crochets qui se fixe à la paroi de l'intestin de l'individu parasité. Promega PCR molecular markers were used (lane M). In order to clarify the taxonomic status of the Taenia saginata-like tapeworm in East Asia, the morphological characteristics of the adult and cysticercus of classical T. saginata (American, Swiss and Poland strains) and the Taiwan Taenia were compared in the present study. The first DNA probe, probe HDP1, is a repetitive sequence that yielded PCR probes specific forT. Although T. solium and T. saginata are very similar, the extraintestinal T. solium infection is far more dangerous and serious. Interestingly, three of the HDP1 monomeric units had the same point mutation in the same nucleotide and at the same position, suggesting that these mutations were not random variations. Morphology (return to top) 2015), North America (Cantey et al., 2014; … Taenia saginata andTaenia solium are the two taeniids of greatest economic and medical importance, causing bovine and porcine cysticercosis and taeniasis in humans. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. saginata gDNA. The scolex of T. saginata has four large suckers but lacks the rostellum and rostellar hooks. The morphologic characteristics of these two parasites are very similar. 6B). Finally, the sensitivity of the multiplex PCR with HDP2-based primers was shown to be 10 pg of DNA when T. saginata, T. solium, and E. granulossusgDNAs were used as templates (data not shown). Copro-antigen detection is very useful in community screening for human taeniosis, particularly for T. solium, but there are no data on copro-antigen detection in pre-patent infection. An internal primer, primer PTs4I1 (5′-ATACTACCAAATCGCAT-3′), was also prepared. A recently developed Western blot assay measures antibody to adult Taenia and thus does not necessarily detect an active infection (43). (B) Locations of probes 5PHDP2, 1PHDP2, and 3PHDP2 within the 3,954-bp sequence of the T. saginata and T. solium HDP2 genomic clone. Hello Medicos, I am Ankita Subhash Lunawat, student of 2nd year BHMS at Ahmednagar Homeopathic Medical College. Design of a T. saginata species-specific PCR with HDP1-based primers.Use of conventional PCR protocols with the primers described above yielded nonspecific results, probably due to the repetitive nature of the sequences and the high degree of complementarity between the forward and reverse primers (see HDP1 and HDP2 sequence analysis). saginata, T. solium, and E. granulossus gDNAs yielded positive but species-specific patterns for each of the three parasites (Fig.11): two bands (of 600 and 170 bp) withT. On an x-ray film of the small bowel, the beef tapeworm Taenia saginata was detected as a long, translucent filling defect (arrows) in the preterminal ileal loop. Beef contains the larval form of this helminth known as cysticercus. The HDP2 sequence was isolated from the recombinant phage by EcoRI digestion (Promega Corporation), yielding a 3,954-bp fragment which was subcloned into the EcoRI site of pBluescript KS+(Stratagene, La Jolla, Calif.). Although the HDP1 genomic representation of 0.4% of T. saginata was very low for satellite DNA, a similarly low percentage had also been found in Caenorhabditis eleganssatellite DNA (21). Humans are infected by. saginata, T. solium, T. taeniformis, E. granulossus, a human, and a calf,T. Infection with the beef tapeworm, Taenia saginata, may cause mild gastrointestinal upset or passage of a motile segment in the stool. Journal of Microbiology & Biology Education, Microbiology and Molecular Biology Reviews. HDP1 and HDP2 sequencing.A designated progressive unidirectional erase strategy (Promega Corporation) was used in order to sequence the T. saginata DNA inserts. With 1 ng of gDNA fromT. In addition, T. solium eggs can infect humans, often giving rise to fatal neurocysticercosis (12, 39).Infections with these cestodes are therefore a serious public health problem in areas of endemicity. These mutations yielded three newScaI recognition sites and one BglI recognition site within the mutated motifs. The PCR could detect 10 pg of T. saginata DNA and yielded the characteristic ladder pattern, but a partial amplification could be observed even with as little as 1 pg of T. saginata DNA. Washington, DC 20036 Nucleotide sequence accession numbers.The HDP1 sequence was assigned accession no. A, B: Taenia spp. PTs4R1 (0.5 μM) was added to the reaction mixture at the 25th cycle. This fact and Southern blot analysis (Fig. Introduction 11 2.2. A negative control without DNA was also included (lane 8). Extraction and sources of DNA.Genomic DNAs (gDNAs) ofT. Characterization of an unusually conserved Alu I highly reiterated DNA sequence family from the honeybee, Cloning and characterization of a highly conserved satellite DNA sequence specific for the phytoparasitic nematode, Development of a serologic assay to detect, Submission, Review, & Publication Processes, Differential Diagnosis of Taenia saginata and Taenia solium Infection by PCR, Copyright © 2000 American Society for Microbiology.

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